Blind deconvolution for thin-layered confocal imaging
نویسندگان
چکیده
منابع مشابه
Blind deconvolution for thin-layered confocal imaging.
We propose an alternate minimization algorithm for estimating the point-spread function (PSF) of a confocal laser scanning microscope and the specimen fluorescence distribution. A three-dimensional separable Gaussian model is used to restrict the PSF solution space and a constraint on the specimen is used so as to favor the stabilization and convergence of the algorithm. The results obtained fr...
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ژورنال
عنوان ژورنال: Applied Optics
سال: 2009
ISSN: 0003-6935,1539-4522
DOI: 10.1364/ao.48.004437